Electromagnetic Field Analysis of Deoxyribonucleic Acid Rolling Circle Amplification in TM010 Resonator

Takeo YOSHIMURA  Takamasa HANAI  Shigeru MINEKI  Jun-ichi SUGIYAMA  Chika SATO  Noriyuki OHNEDA  Tadashi OKAMOTO  Hiromichi ODAJIMA  

Publication
IEICE TRANSACTIONS on Electronics   Vol.E99-C   No.11   pp.1287-1294
Publication Date: 2016/11/01
Online ISSN: 1745-1353
Type of Manuscript: PAPER
Category: Microwaves, Millimeter-Waves
Keyword: 
dielectric-loss,  conductive-loss,  heating resonator,  solid-state oscillator,  DNA amplification,  

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Summary: 
Microwave heating is expected to increase the yield of product, to decrease the reaction time, and to discover the new reaction system. The Rolling Circle Amplification (RCA) is an enzymatic synthesis method of deoxyribonucleic acid (DNA) strands with repeated sequence of a circulate template-DNA. In previous study, controlled microwave heating accelerated the maximum 4-fold compared with the conventional condition. Further, we indicated that the selectively heat of some buffer components by microwave irradiation induced the acceleration of RCA. The purpose of this research is to clarify the relationship between the microwave heating and buffer components. The understanding of role of ion-containing buffer components under microwave will be able to control the microwave-assisted enzymatic reaction. We studied the relation between the microwave power loss and RCA components via dielectric measurements, cavity resonator feature measurement, and electromagnetic simulation. Electromagnetic simulation of the TM010 cavity showed that the sample tube was heated only by an electric field. The buffer containing ions of the RCA components was selectively heated via microwave irradiation in the TM010 cavity resonator.