Label-Free and Noninvasive Monitoring of Cell Differentiation on Spheroid Microarray

Hidenori OTSUKA  Masako NAGAMURA  Akie KANEKO  Koichi KUTSUZAWA  Toshiya SAKATA  

Publication
IEICE TRANSACTIONS on Electronics   Vol.E96-C   No.3   pp.353-357
Publication Date: 2013/03/01
Online ISSN: 1745-1353
DOI: 10.1587/transele.E96.C.353
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Section on Recent Progress in Molecular and Organic Devices)
Category: 
Keyword: 
3D cell culture,  spheroid,  bovine articular cartilage,  glycosaminoglycan (GAG),  field effect transistor (FET),  

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Summary: 
A two-dimensional microarray of ten thousand (100100) chondrocyte-spheroids was successfully constructed with a 100-µm spacing on a micropatterned gold electrodes that were coated with poly(ethylene glycol) (PEG) hydrogels. The PEGylated surface as a cytophobic region was regulated by controlling the gel structure through photolithography. In this way, a PEG hydrogel was modulated enough to inhibit outgrowth of chondrocytes from cell adhering region in the horizontal direction. These structural control of PEG hydrogel was critical for inducing formation of three-dimensional chondrocyte condensations (spheroids) within 24 hours. We report noninvasive monitoring of the cellular functional change at the cell membrane using a chondrocyte-based field effect transistor (FET), which is based on detection of extracellular potential change induced as a result of the interaction between extracellular matrix (ECM) protein secreted from spheroid and substrate at the cell membrane. The interface potential change at the cell membrane/gate insulator interface can be monitored during the uptake of substrate without any labeling materials. Our findings on the time course of the interface potential would provide important information to understand the uptake kinetics for cellular differentiation.