Low Voltage Pulse Application to Biological Cells

Hidenori OTSUKA
Saya OKIMURA
Masako NAGAMURA
Daisuke MATSUKUMA
Koichi KUTSUZAWA
Naoki MATSUDA
Hirotaka OKABE

Publication
IEICE TRANSACTIONS on Electronics   Vol.E96-C    No.3    pp.348-352
Publication Date: 2013/03/01
Online ISSN: 1745-1353
DOI: 10.1587/transele.E96.C.348
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Section on Recent Progress in Molecular and Organic Devices)
Category: 
Keyword: 
pulsed electric fields (PEF),  low voltage pulse,  layer by layer (LBL),  cell viability,  

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Summary: 
As an application of low electric field to biomedical engineering, this paper attempts to study the dose-effect of biological effects caused by msPEF with experiments on HeLa cells. MTT assay was used to trace the cell electroporation and examine cell viability. It is observed that with the increasing electric field intensity and pulse numbers, IRE effects will occur successively.