In Situ Observation of Reduction Behavior of Cytochrome c Adsorbed on Glass Surface by Slab Optical Waveguide Spectroscopy

Naoki MATSUDA  Hirotaka OKABE  Masaki FUJII  Masayoshi MATSUI  Yusuke AYATO  Akiko TAKATSU  Kenji KATO  

Publication
IEICE TRANSACTIONS on Electronics   Vol.E94-C   No.2   pp.170-175
Publication Date: 2011/02/01
Online ISSN: 1745-1353
DOI: 10.1587/transele.E94.C.170
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Section on Recent Progress in Molecular and Organic Devices)
Category: 
Keyword: 
slab optical waveguide spectroscopy,  cytochrome c,  adsorption,  reduction reaction,  solid/liquid interface,  

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Summary: 
In situ observation of the adsorption process and the states of cytochrome c on glass/solution interfaces, and the functionality of the reduction reaction of adsorbed cytochorome c were performed by using slab optical waveguide (SOWG) spectroscopy. The peak position of the absorption band of cytochorome c adsorbed on a bare glass surface was almost the same as that of that in solution. The cytochorome c adsorbed on glass/solution interface was reduced by sodium dithionite solution. The adsorbed cytochorome c was still maintained its functionality after immobilization.