In Situ Observation of Time Dependent Electrochemical Activity of Cytochrome c at Bare Indium-Tin-Oxide Electrodes by Cyclic Voltammetry and Slab Optical Waveguide Spectroscopy

Yusuke AYATO  Akiko TAKATSU  Kenji KATO  Naoki MATSUDA  

Publication
IEICE TRANSACTIONS on Electronics   Vol.E91-C   No.12   pp.1899-1904
Publication Date: 2008/12/01
Online ISSN: 1745-1353
DOI: 10.1093/ietele/e91-c.12.1899
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Section on The Forefront of 21st Century Organic Molecular Electronics)
Category: Bioelectronics
Keyword: 
protein,  electrochemistry,  cytochrome c,  slab optical waveguide (SOWG) spectroscopy,  in situ,  

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Summary: 
In situ observation of electrochemical activity and time dependent characteristics of cytochrome c (cyt c) was carried out in 0.01 M phosphate buffered saline (PBS, pH 7.4) containing 20 µM cyt c solutions at bare indium-tin-oxide (ITO) electrodes by using a cyclic voltammetry (CV) and a slab optical waveguide (SOWG) spectroscopy. The bare ITO electrodes could retain the electrochemical activity of cyt c in the PBS solutions, indicating the great advantage of using ITO electrodes against other electrode materials, such as gold (Au). The CV curves and simultaneously observed the time-resolved SOWG absorption spectra in the consecutive cycles implied that the cyt c molecules could retain its own electrochemical function for a long time.