Conducting Polymer Based Nucleic Acid Sensor for Environment Monitoring

Bansi Dhar MALHOTRA  Nirmal PRABHAKAR  Pratima R. SOLANKI  

IEICE TRANSACTIONS on Electronics   Vol.E91-C   No.12   pp.1889-1893
Publication Date: 2008/12/01
Online ISSN: 1745-1353
DOI: 10.1093/ietele/e91-c.12.1889
Print ISSN: 0916-8516
Type of Manuscript: INVITED PAPER (Special Section on The Forefront of 21st Century Organic Molecular Electronics)
nucleic acid sensor,  electrochemical,  polyaniline,  perchlorate,  arsenic trioxide,  

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Nucleic acid sensor based on polyaniline has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO- 4) doped polyaniline (PANI) film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) chemistry. These dsCT-DNA-PANI/ITO and PANI/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, and Fourier-transform-infra-red (FTIR) measurements. This disposable dsCT-DNA-PANI/ITO bioelectrode is stable for about four months, can be used to detect arsenic trioxide (0.1 ppm) in 30 s.