Photosynthetic Activity Measurement of Plants Using Photoacoustic Spectroscopy Combined with Confocal Scanning Microscopy

Hideo KOJIMA  Masahiro TAWATA  Teruhiro TAKABE  Hiroshi SHIMOYAMA  

IEICE TRANSACTIONS on Electronics   Vol.E83-C    No.7    pp.1142-1148
Publication Date: 2000/07/25
Online ISSN: 
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Issue on Organic Molecular Electronics for the 21st Century)
Category: Optics and Bio Electronics
photoacoustic spectroscopy,  photosynthesis,  confocal scanning laser microscopy,  

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Photoacoustic spectroscopy (PAS) has recently received much attention especially for plant photosynthesis research, because this technique is capable of performing non-destructive measurement without any pre-treatment of specimens. So far we have developed a PAS system equipped with an open photoacoustic cell (OPC), which allows in situ and in vivo measurements of plant photosynthesis of intact undetached leaves. In this study, we have measured photosynthesis reaction using OPC and developed a Confocal Scanning Photoacoustic Microscopy (CSPAM) system, in which PAS is combined with confocal scanning laser microscopy. The system allows simultaneous measurement of acoustic signal and another signal such as fluorescence, and also gives two- and three- dimensional intensity distributions of these signals, thereby giving two- and three- dimensional information about photosynthetic activity of plants.