Formation of Black Membrane Using a Microfabricated Orifice

Masao WASHIZU  Seiichi SUZUKI  Osamu KUROSAWA  Hideaki KURAHASHI  Akira KATOH  

Publication
IEICE TRANSACTIONS on Electronics   Vol.E78-C   No.2   pp.157-161
Publication Date: 1995/02/25
Online ISSN: 
DOI: 
Print ISSN: 0916-8516
Type of Manuscript: Special Section PAPER (Special Issue on Micromachines and Micro Electro Mechanical Systems)
Category: 
Keyword: 
microfabrication,  black membrane,  biological membrane,  channel,  silane coupler,  

Full Text: PDF>>
Buy this Article




Summary: 
A black membrane is a biological-membrane analogue, i.e. a phospholipid bilayer membrane, artificially formed on an orifice immersed in water. It is used to investigate the properties of the membrane itself and channels embedded therein. In this paper, microfabrication techniques are applied to fabricate the orifice, and a glass substrate is isotropically etched to define the orifice geometry. The periphery of the orifice was patterned with aminosilane to anchor the membrane. The remainder part was coated with fluorosilane to make the surface hydrophobic and to prevent adsorption of channel-forming molecules. We demonstrated experimentally that a stable and reproducible membrane is easily obtainable using the orifice.