An Application of the Optimal Control Strategy for Artificial Production of Protein on Messenger RNA

Hirohumi HIRAYAMA  Norio TAKEUCHI  Yuzou FUKUYAMA  

Publication
IEICE TRANSACTIONS on Fundamentals of Electronics, Communications and Computer Sciences   Vol.E76-A   No.12   pp.2076-2081
Publication Date: 1993/12/25
Online ISSN: 
DOI: 
Print ISSN: 0916-8508
Type of Manuscript: Special Section LETTER (Special Section of Letters Selected from the 1993 IEICE Fall Conference)
Category: 
Keyword: 
optimal control,  protein synthesis,  messenger RNA,  robozome,  

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Summary: 
The regulatory mechanism of protein synthesis on a messenger RNA was analyzed from view point of the optimal control and discussed about availability for artificial production of peptide and protein. The transient movements of a ribozome through a messenger RNA with its production of peptide was based on the theory proposed by Gordon (1968). The optimal state of total process was defined as the state at which the time dependent change of each process of peptide synthesis has been minimized during a given time interval. This biological problem was converted into mathematical one by setting state variables and utilizing the optimal control theory with the help of Hamiltonian function. The first process of transition of a ribozome on a messenger RNA showed the largest change and with progress of state, the magnitude of change of each process decreased and became a simpler pattern. The effect of weighting coefficient relating with individual process was not confined only to its proper process but extended to all other processes. Each process was affected from all other processes. These were manifestations of effective and rational control strategies particularly for regulation of the sequential reaction in peptide synthesis. Such results were originated in the operation of the optimal control. By simulating physiological experimental data, it is possible to predict at what process and at what degree, the synthesis is regulated in order to achieve the optimal synthesis state. By analyzing the optimal synthesis process in combination with physiological experimental data, it would be possible to create artificial peptide and protein.