In situ Observation of Immobilization of Cytochrome c into Hydrophobic DNA Nano-Film

Naoki MATSUDA  Hirotaka OKABE  Ayako OMURA  Miki NAKANO  Koji MIYAKE  Toshihiko NAGAMURA  Hideki KAWAI  

IEICE TRANSACTIONS on Electronics   Vol.E102-C   No.6   pp.471-474
Publication Date: 2019/06/01
Online ISSN: 1745-1353
DOI: 10.1587/transele.2018FUS0004
Type of Manuscript: BRIEF PAPER
Hydrophobic DNA nano-film,  cytochrome c,  immobilization,  slab optical waveguide spectroscopy,  

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Hydrophobic DNA (H-DNA) nano-film was formed as the surface modifier on a thin glass plate working as a slab optical waveguide (SOWF). Cytochrom c (cytc) molecules were immobilized from aqueous solution with direct contacting to the H-DNA nano-film for 30 minutes. From SOWG absorption spectral changes during automated solution exchange (SE) processes, it was found that about 28.1% of cytc molecules was immobilized in the H-DNA nano-film with keeping their reduction functionality by reducing reagent.