In situ Observation of Capturing BTB Molecules from Aqueous Solutions with Hydrophobic DNA Nano-Film

Naoki MATSUDA  Hirotaka OKABE  Ayako OMURA  Miki NAKANO  Koji MIYAKE  Toshihiko NAGAMURA  Hideki KAWAI  

Publication
IEICE TRANSACTIONS on Electronics   Vol.E102-C   No.2   pp.203-206
Publication Date: 2019/02/01
Online ISSN: 1745-1353
DOI: 10.1587/transele.2018OMS0018
Type of Manuscript: BRIEF PAPER
Category: 
Keyword: 
hydrophobic DNA nano-film,  bromothymol blue,  immobilization,  slab optical waveguide spectroscopy,  in situ observation,  

Full Text: PDF(469.6KB)
>>Buy this Article


Summary: 
Hydrophobic DNA (H-DNA) nano-film was formed on a thin glass plate of 50μm thick working as a slab optical waveguide. Bromothymol blue (BTB) molecules were immobilized from aqueous solution with direct contacting to the H-DNA nano-film for 20 minutes. From changes in absorption spectra observed with slab optical wave guide (SOWG) during automated solution exchange (SE) processes for 100 times, it was found that about 95% of bromothymol blue (BTB) molecules was immobilized in the H-DNA nano-film with keeping their functionality of color change responsible to pH change in the solution.