Biodegradable Neural Cell Culture Sheet Made of Polyanhydride thin Film with Micro-Trench Structures

Yuki NAKAMURA  Satomitsu IMAI  

Publication
IEICE TRANSACTIONS on Electronics   Vol.E102-C   No.2   pp.164-167
Publication Date: 2019/02/01
Online ISSN: 1745-1353
DOI: 10.1587/transele.2018OMS0015
Type of Manuscript: BRIEF PAPER
Category: 
Keyword: 
culture sheet,  polyanhydride,  MIMIC,  SU-8,  thiol-ene polymerization,  

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Summary: 
Technological developments in direction control of axonal outgrowth are a must for advances in regenerative medicine of the nervous system. In order to solve the problem, we fabricate a new neural cell culture sheet by applying the soft lithography technique to micro-patterning of the extracellular matrix and using thin-film biodegradable polymer for the scaffold. Micro-trenches were coated with Dulbecco's phosphate-buffered saline (-) containing laminin, using micro-molding in capillaries (MIMIC), a soft lithography technique. Biodegradable thin films with micro-trenches were fabricated by UV-curing a polyanhydride solution covering the negative SU-8 mold through thiol-ene polymerization. Both approaches were performed conveniently, rapidly, and accurately. It is thought that these techniques are excellent in terms of convenience and high speed, and can contribute greatly to regenerative medicine.