Detection of Human Immunoglobulin G by Transmission Surface Plasmon Resonance Using the In Situ Gold Nanoparticle Growth Method

Theerasak JUAGWON  Chutiparn LERTVACHIRAPAIBOON  Kazunari SHINBO  Keizo KATO  Toemsak SRIKHIRIN  Tanakorn OSOTCHAN  Akira BABA  

IEICE TRANSACTIONS on Electronics   Vol.E102-C   No.2   pp.125-131
Publication Date: 2019/02/01
Online ISSN: 1745-1353
DOI: 10.1587/transele.2018OMP0004
Type of Manuscript: Special Section PAPER (Special Section on Recent Progress in Organic Molecular Electronics and Biotechnology)
transmission surface plasmon,  gold nanoparticle,  immunosensor,  

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In this work, we report the in situ growth of gold nanoparticles (AuNPs) for the improvement of a transmission surface plasmon resonance (T-SPR) sensor to detect human immunoglobulin G (IgG). Human IgG was immobilized on an activated self-assembled monolayer of 11-mercaptoundecanoic on a gold-coated grating substrate. The T-SPR system was also used to monitor the construction of sensor chips as well as the binding of IgG and anti-IgG conjugated with AuNPs. After specific adsorption with IgG, the T-SPR signal was further enhanced by the in situ growth of AuNPs bound with anti-IgG. Using AuNP conjugation and in situ growth of bound AuNPs, the sensitivity of the IgG immunosensor was improved by two orders of magnitude compared with that without conjugated AuNPs.